Chondrogenic potential of human articular chondrocytes and skeletal stem cells: a comparative study

Regenerative medicine strategies have increasingly focused on skeletal stem cells (SSCs), in response to concerns such as donor site morbidity, dedifferentiation and limited lifespan associated with the use of articular chondrocytes for cartilage repair. The suitability of SSCs for cartilage regeneration, however, remains to be fully determined. This study has examined the chondrogenic potential of human STRO-1-immunoselected SSCs (STRO-1+ SSCs), in comparison to human articular chondrocytes (HACs), by utilising two bioengineering strategies, namely ‘‘scaffold-free’’ three-dimensional(3-D) pellet culture and culture using commercially available, highly porous, 3-D scaffolds with interconnected pore networks. STRO-1+ SSCs were isolated by magnetic-activated cell sorting from bone marrow samples of haematologically normal osteoarthritic individuals following routine hip replacement procedures. Chondrocytes were isolated by sequential enzymatic digestion of deep zone articular cartilage pieces dissected from femoral heads of the same individuals. After expansion in monolayer cultures, the harvested cell populations were centrifuged to form high-density 3-D pellets and also seeded in the 3-D scaffold membranes, followed by culture in serum-free chondrogenic media under static conditions for 21 and 28 days, respectively. Chondrogenic differentiation was determined by gene expression,histological and immunohistochemical analyses. Robust cartilage formation and expression of hyaline cartilage-specific markers were observed in both day-21 pellets and day-28 explants generated using HACs. In comparison, STRO-1+ SSCs demonstrated significantly lower chondrogenic differentiation potential and a tendency for hypertrophic differentiation in day-21 pellets. Culture of STRO-1+ SSCs in the 3-D scaffolds improved the expression of hyaline cartilage-specific markers in day-28 explants, however, was unable to prevent hypertrophic differentiation of the SSC population. The advantages of application of SSCs in tissue engineering are widely recognised; the results of this study, however, highlight the need for further development of cell culture protocols that may otherwise limit the application of this stem cell population in cartilage bioengineering strategies

Distal subgaleal-peritoneal shunt migration into the abdominal wall with subsequent formation of a pre-peritoneal pseudocyst: a rare complication.

Distal ventriculo-peritoneal shunt migration and extra-peritoneal CSF pseudocyst formation are unusual complications of shunt placement. We present a 65-year-old-female who received a subgaleal-peritoneal shunt to decompress a post-surgical subgaleal fluid collection. Eight weeks later, shunt series showed tight coiling of the distal catheter, and operative exploration found the distal shunt tip to have migrated superficial to the rectus sheath, where it had become encapsulated in a pre-peritoneal CSF pseudocyst. Migration of the distal catheter into the abdominal wall was likely due to local inflammation of the inner surface of the abdomen, with pressure from intestinal peristaltic movements and intra-abdominal pressure, and continued inflammation at the distal catheter tip may have caused formation of a pre-peritoneal CSF pseudocystic dilatation. To date, this is the first reported case of distal shunt migration into the abdominal wall with subsequent formation of an extra-peritoneal pseudocyst and represents a rare event in the surgical management of peritoneal shunts

Advanced Magnetic Resonance Imaging in Glioblastoma: A Review

INTRODUCTION In 2017, it is estimated that 26,070 patients will be diagnosed with a malignant primary brain tumor in the United States, with more than half having the diagnosis of glioblas- toma (GBM).1 Magnetic resonance imaging (MRI) is a widely utilized examination in the diagnosis and post-treatment management of patients with glioblastoma; standard modalities available from any clinical MRI scanner, including T1, T2, T2-FLAIR, and T1-contrast-enhanced (T1CE) sequences, provide critical clinical information. In the last decade, advanced imaging modalities are increasingly utilized to further charac- terize glioblastomas. These include multi-parametric MRI sequences, such as dynamic contrast enhancement (DCE), dynamic susceptibility contrast (DSC), diffusion tensor imaging (DTI), functional imaging, and spectroscopy (MRS), to further characterize glioblastomas, and significant efforts are ongoing to implement these advanced imaging modalities into improved clinical workflows and personalized therapy approaches. A contemporary review of standard and advanced MR imaging in clinical neuro-oncologic practice is presented

The enhancement of the aqueous solubility of ritonavir via formulation of a drug-phospholipid complex

Objective: To evaluate the enhancement of aqueous solubility of a poorly water soluble drug ritonavir by forming its complex with a phospholipid (Phospholipon®90H)

EVALUATION OF PROCESS CAPABILITY IN MANUFACTURE OF ANTIHYPERTENSIVE TABLETS 10 MG

Background of the Study: A Process Capability Study is a formal procedure for undertaking a systematic investigation to reliably assess a process ability to consistently meet a specific requirement. Its indices are to measure the inherent variability of a process and thus to reflect its performance.Objective: The present study describes the evaluation of Process capability in the manufacture of Anti-hypertensive tablets 10 mg.Materials and Methods: This project focuses on Process Capability Study that had been applied in Pharmaceutical industry which includes selection of critical parameters, data collection, study on process capability and data evaluation. In this process capability study the critical process parameters were identified and evaluated by challenging its lower and upper release specifications. Here many statistical process control tools along with Minitab-16 software were used to find the capability of this manufacturing process.Results and Discussion: The discovered results for Ppk (Process performance index), tolerance limit and Standard deviation (overall) are 2.25, 40 and 2.949, which advocates that the process meets the criteria required for capability. Process capability ensures that processes are fit for industry company specification while reduce the process variation and important in achieving product quality characteristic. This capability study should be employed in the industry before the batch is made commercial. It acts as a cost effective approach that can reduce the time taken for inspection.Conclusion: In this research work the current capability of the process is predicted and the process is found to be capable.Keywords: Process capability, Specifications, Critical parameters

Analysis of Qos Aware Cloud Based Routing for Improved Security

The recent advances and the convergence of micro electro-mechanical systems technology, integrated circuit technologies, microprocessor hardware and Nano-technology, wireless communications, Ad-hoc networking routing protocols, distributed signal processing, and embedded systems have made the concept of Wireless Sensor Networks (WSNs). Sensor network nodes are limited with respect to energy supply, restricted computational capacity and communication bandwidth. Most of the attention, however, has been given to the routing protocols since they might differ depending on the application and network architecture. To prolong the lifetime of the sensor nodes, designing efficient routing protocols is critical. Even though sensor networks are primarily designed for monitoring and reporting events, since they are application dependent, a single routing protocol cannot be efficient for sensor networks across all applications. In this paper, we analyze the design issues of sensor networks and present a classification and comparison of routing protocols. This comparison reveals the important features that need to be taken into consideration while designing and evaluating new routing protocols for sensor networks. A reliable transmission of packet data information, with low latency and high energy-efficiency, is truly essential for wireless sensor networks, employed in delay sensitive industrial control applications. The proper selection of the routing protocol to achieve maximum efficiency is a challenging task, since latency, reliability and energy consumption are inter-related with each other. It is observed that, Quality of Service (QoS) of the network can improve by minimizing delay in packet delivery, and life time of the network, can be extend by using suitable energy efficient routing protocol

Connecting protein and mRNA burst distributions for stochastic models of gene expression

The intrinsic stochasticity of gene expression can lead to large variability in protein levels for genetically identical cells. Such variability in protein levels can arise from infrequent synthesis of mRNAs which in turn give rise to bursts of protein expression. Protein expression occurring in bursts has indeed been observed experimentally and recent studies have also found evidence for transcriptional bursting, i.e. production of mRNAs in bursts. Given that there are distinct experimental techniques for quantifying the noise at different stages of gene expression, it is of interest to derive analytical results connecting experimental observations at different levels. In this work, we consider stochastic models of gene expression for which mRNA and protein production occurs in independent bursts. For such models, we derive analytical expressions connecting protein and mRNA burst distributions which show how the functional form of the mRNA burst distribution can be inferred from the protein burst distribution. Additionally, if gene expression is repressed such that observed protein bursts arise only from single mRNAs, we show how observations of protein burst distributions (repressed and unrepressed) can be used to completely determine the mRNA burst distribution. Assuming independent contributions from individual bursts, we derive analytical expressions connecting means and variances for burst and steady-state protein distributions. Finally, we validate our general analytical results by considering a specific reaction scheme involving regulation of protein bursts by small RNAs. For a range of parameters, we derive analytical expressions for regulated protein distributions that are validated using stochastic simulations. The analytical results obtained in this work can thus serve as useful inputs for a broad range of studies focusing on stochasticity in gene expression

GERIATRIC HEALTH CARE THROUGH NUTRACEUTICALS OF MORINDA CITRIFOLIA L.: A REVIEW

Ageing (Jara) is a natural process and an inevitable phenomenon in life. Ageing not only brings about a continued generalized involution of the biosystem, it also predisposes in aged individual to set of diseases and disorder warranting special medical care. Problems of geriatric age group health problems are joint problems, impairment of special senses, cardio vascular disease, hypothermia, cancer, prostate enlargement, diabetes & accidental falls psychological problems. Noni is the common name for Morinda citrifolia L and is also called Indian Mulberry. Morinda species has been described in Ayurveda as the name of Akshiki phala, Ashyuka and also used in folk remedies by traditionally for over 2000 years, and is reported to have a broad range of therapeutic effects, including antibacterial, antiviral, antifungal, antitumor, antihelmin, analgesic, hypotensive, anti-inflammatory, and immune enhancing effects. The aim of this study is to explore the importance of Morinda citrifolia to reveal the nutritional and medicinal value of the Noni plant, and to summarize scientific evidence that supports it geriatric health care through its neutraceuticals such as vit C, flavanoids, tannins and antioxidants

Poincar\'e Invariant Quantum Field Theories With Twisted Internal Symmetries

Following up the work of [1] on deformed algebras, we present a class of Poincar\'e invariant quantum field theories with particles having deformed internal symmetries. The twisted quantum fields discussed in this work satisfy commutation relations different from the usual bosonic/fermionic commutation relations. Such twisted fields by construction are nonlocal in nature. Despite this nonlocality we show that it is possible to construct local interaction Hamiltonians which satisfy cluster decomposition principle and are Lorentz invariant. We further illustrate these ideas by considering global SU(N) symmetries. Specifically we show that twisted internal symmetries can significantly simplify the discussion of the marginal deformations (\beta-deformations) of the N=4 SUSY theories.Comment: 27 pages, Typos Corrected, Text and Conclusions Unchanged, Version published in JHE